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Boster
For quantitative detection of human IL-8 in cell culture supernates, serum and plasma(heparin, EDTA, citrate).
Human
There is no detectable cross-reactivity with other relevant proteins.
Human IL-8Protein name: Interleukin-8Gene Full Name: chemokine (C-X-C motif) ligand 8abbreviation: CXCL8Background: Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor(MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils. IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications. The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members(platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside. Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form. ID:P10145 ID:3576
(Ala-IL-8)77| (Ser-IL-8)72| 3 10C| 3-10C| AMCF I| AMCF-I| AMCFI| b ENAP| B-ENAP| Beta-thromboglobulin-like protein| C X C motif chemokine 8| C-X-C motif chemokine 8| Chemokine (C X C motif) ligand 8| CXCL8 | Emoctakin| GCP 1| GCP-1| GCP/IL-8 protein I| GCP/IL-8 protein II| GCP/IL-8 protein III| GCP/IL-8 protein IV| GCP/IL-8 protein V| GCP/IL-8 protein VI| GCP1 | Granulocyte chemotactic protein 1| IL 8| IL-8| IL-8(1-77)| IL-8(9-77)| IL8| IL8/NAP1 form I| IL8/NAP1 form II| IL8/NAP1 form III| IL8/NAP1 form IV| IL8/NAP1 form V| IL8/NAP1 form VI| IL8_HUMAN| Interleukin 8| K60| LECT| LUCT| Lymphocyte derived neutrophil activating factor| Lymphocyte-derived neutrophil-activating factor| LYNAP| MDNCF| MDNCF-b| MDNCF-c| MONAP| Monocyte derived neutrophil activating peptide| Monocyte derived neutrophil chemotactic factor| Monocyte-derived neutrophil chemotactic factor| Monocyte-derived neutrophil-activating peptide| NAF| NAP 1| NAP-1| NAP1| Neutrophil activating peptide 1| Neutrophil activating protein 1| Neutrophil-activating factor| Neutrophil-activating protein 1| Protein 3 10C| Protein 3-10C| SCYB8| Small inducible cytokine subfamily B member 8| T cell chemotactic factor| T-cell chemotactic factor| TSG 1| TSG-1| TSG1
Nativ and recombinant human IL-8
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
E.coli, S28-S99
<1pg/ml
15.6pg/ml-1000pg/ml(cellculturesupernates)7.8pg/ml-500pg/ml(serum,plasma)
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|115|357|616|176|392|649 Standard deviation|6.67|17.5|41.3|13.38|21.17|45.43 CV(%)|5.8|4.9|6.7|7.6|5.4|7
15-20
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s human IL-8 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-8 has been precoated onto 96-well plates. Standards(E.coli, S28-S99) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-8 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-8 amount of sample captured in plate.
Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml human IL-8 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human IL-8 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000 O.D.|0.003|0.170|0.321|0.594|1.121|1.878|2.552|2.779
Catalog number|Description|Quantity-|96-well plate precoated with anti- human IL-8 antibody|1ST0000-10|Lyophilized recombinant human IL-8 standard|10ng/tubex2AR1107|Biotinylated anti- human IL-8 antibody|130ul(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)AR1106-1|Sample diluent buffer|30mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Menge | 1x96 Wells |
---|---|
Hersteller | Boster |
Herstellerland | USA |
Sensitivity | <1pg/ml |
Specific Against | Human |
Antigen Name | Interleukin-8 (CXCL8) |
Detection Range | 15.6pg/ml-1000pg/ml(cellculturesupernates)7.8pg/ml-500pg/ml(serum,plasma) |
Alias | (Ala-IL-8)77, (Ser-IL-8)72, 3 10C, 3-10C, AMCF I, AMCF-I, AMCFI, b ENAP, B-ENAP, Beta-thromboglobulin-like protein, C X C motif chemokine 8, C-X-C motif chemokine 8, Chemokine (C X C motif) ligand 8, CXCL8 , Emoctakin, GCP 1, GCP-1, GCP |