For the quantitative determination of Human IL-4R / CD124 concentration in serum and cell culture supernates.
The average concentration of IL4R in 10 normal human serum is 995.21 +/- 185.18 pg/mL ranging from 742.10 to 1257.58 pg/mL. HepG2 cells (0.2 x 10E5 cells/mL) were cultured 3 days. An aliquot of the cell culture supernate was removed, assayed for levels of natural DLL1, and measured 12 pg/mL.
This assay recognizes both recombinant and natural Human IL4R. The factors listed below were prepared at 100 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
IL4RAntigene: 10402-H08Hcapture antibody type: rabbit mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Detection Range||3.125-200 pg/ml|
|Alias||IL-4R / CD124|