Human IL-37/IL1F7 PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human IL-37 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

human IL-37
Protein name: Interleukin-37
Gene Full Name: interleukin 37
abbreviation: IL37
Background: Interleukin 37(IL-37) also named IL1F7, is a protein that in humans is encoded by the IL1F7 gene. The protein encoded by this gene is a member of the interleukin 1 cytokine family. And it is mapped to 2q13. In vitro expression analysis showed that IL-37 was a secretory protein. And IL-37 could play an important role in the link between innate and adaptive immunity and may be useful for tumor immunotherapy. Meanwhile, IL-37 inhibits the production of inflammatory cytokines in peripheral blood mononuclear cells of patients with systemic lupus erythematosus. In addition, IL-37 can emerged as a dual-function cytokine with intra- and extracellular properties for suppressing innate inflammation. Furthermore, IL-37 is a new anti-inflammatory cytokine that plays an important role in protecting against tissue injury during infections via limiting immune and inflammatory reactions.
ID:Q9NZH6
ID:27178

alias

FIL1|FIL1 zeta|FIL1(ZETA)|FIL1Z|IL 1 zeta|IL 1F7|IL 1F7b (IL 1H4, IL 1H, IL 1RP1)|IL 1H4|IL 1RP1|IL 1X|IL 1X protein|IL-1 zeta|IL-1F7|IL-1H|IL-1H4|IL-1RP1|IL-1X|IL-37|IL1F7 (canonical product IL 1F7b)|IL1F7|IL1F7_HUMAN|IL1H4|IL1RP1|Interleukin 1 family member 7|interleukin 1 family, member 7 (zeta)|Interleukin 1 homolog 4|Interleukin 1 related protein|Interleukin 1 superfamily z|Interleukin 1 zeta|Interleukin 37|Interleukin-1 family member 7|Interleukin-1 homolog 4|Interleukin-1 zeta|Interleukin-1-related protein|Interleukin-23

Origin

Nativ and recombinant human IL-37

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

E.coli, V46-D218

sensitivity

<10pg/ml

detection range

15.6pg/ml-1000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|178|304|569|216|365|610
Standard deviation|7.3|11.86|30.2|12.3|15.33|39.04
CV(%)|4.1|3.9|5.3|5.7|4.2|6.4

TMB(tetramethylbenzidine) incubation time

25-30

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human IL-37 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-37 has been precoated onto 96-well plates. Standards(E.coli, V46-D218) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-37 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-37 amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml human IL-37 standard solutions into the pre-coated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. We recommend that each human IL-37 standard solution and each sample is measured in duplicate.

tipical result

Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000 O.D.|0.004|0.069|0.135|0.230|0.495|0.905|1.721|2.550

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human IL-37 antibody|1
ST0000-10|Lyophilized recombinant human IL-37 standard|10ng/tubex2
AR1107|Biotinylated anti- human IL-37 antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4