Human IL-34 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human IL-34 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of IL-34 in Human serum, plasma and cell culture media. An antibody specific for Human IL-34 was coated on a 96-well plate. Standards and samples are added to the wells and any IL-34 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human IL-34 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of IL-34 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Inflammation;

specific against

Human

detection target

Interleukin-34(IL-34)
Background: Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N-linked glycosylation. IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen. The receptor for IL-34 is colony-stimulating factor 1 receptor.  IL-34 stimulates monocyte proliferation. In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes. In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures.

alias

C16orf77, IL-34

sensitivity

2 pg/ml

detection range

3.9-250 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

Q6ZMJ4