Human IL-31 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human IL-31 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of IL-31 in Human serum, plasma and cell culture media. An antibody specific for Human IL-31 was coated on a 96-well plate. Standards and samples are added to the wells and any IL-31 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human IL-31 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of IL-31 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Inflammation;

specific against

Human

detection target

Interleukin-31(IL-31)
Background: Interleukin 31 (IL-31) is a secreted, T-cell-derived, 24-kDa, short-chain member of the alpha-helical family of cytokines. The mature region is 141 amino acids (aa) in length and exhibits four alpha-helices.  The receptor for IL-31 is found on keratinocytes and monocytes. It is a heterodimer composed of the Oncostatin M receptor and a novel gp130-related molecule. IL-31 may be involved in pruritis, or skin itching. IL-31 signals via a receptor complex that is composed of IL-31 receptor A (IL31RA) and oncostatin M receptor subunits. These receptor subunits are expressed in activated monocytes and in unstimulated epithelial cells.

alias

IL-31

sensitivity

2 pg/ml

detection range

4.9-1200 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

Q6EBC2