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Boster
For quantitative detection of human IL-3 in cell culture supernates, serum and plasma( heparin, EDTA, citrate).
Human
There is no detectable cross-reactivity with other relevant proteins.
Human IL-3Protein name: Interleukin-3Gene Full Name: interleukin 3abbreviation: IL3Background: The gene IL-3 encodes interleukin 3, a hematopoietic colony-stimulating factor(CSF) that is capable of supporting the proliferation of a broad range of hematopoietic cell types. Interleukin-3(IL-3), a protein of 140 amino acids, is chemically synthesized by means of an automated peptide synthesizer and is shown to have the biological activities attributed to native IL-3. The cDNA sequence for murine interleukin-3, one of the colony stimulating factors that regulate haematopoiesis, codes for a polypeptide of 166 amino acids including a putative signal peptide. The mouse IL 3 gene is located on chromosome 11. The human gene encoding IL 3 is tandemly arrayed on the long arm of chromosome 5. The standard product used in this kit is recombinant human IL-3, consisting of 133 amino acids with the molecular mass of 15KDa. ID:P08700 ID:3562
Burst promoting activity| Colony stimulating factor multiple| Eosinophil colony stimulating factor| Hematopoietic growth factor| IL 3| IL-3| IL3| IL3_HUMAN| Interleukin 3| Interleukin-3| Mast cell growth factor| MCGF| Multi CSF| Multilineage colony stimulating factor| Multipotential colony stimulating factor| Multipotential colony-stimulating factor| P cell stimulating factor| P-cell-stimulating factor| Thy1 inducing factor
Nativ and recombinant human IL-3
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
E.coli,A20-F152
<1pg/ml
15.6pg/ml-1000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|95|323|590|108|372|625 Standard deviation|4.56|12.6|21.83|8.75|24.18|33.75 CV(%)|4.8|3.9|3.7|8.1|6.5|5.4
15-20
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s human IL-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-3 has been precoated onto 96-well plates. Standards (E.coli,A20-F152) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-3 amount of sample captured in plate.
Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml human IL-3 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma( heparin, EDTA, citrate) to each empty well. See モSample Dilution Guidelineヤ above for details. It is recommended that each human IL-3 standard solution and each sample be measured in duplicate.
Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000 O.D.|0.104|0.148|0.189|0.261|0.456|0.762|1.223|1.982
Catalog number|Description|Quantity-|96-well plate precoated with anti- human IL-3 antibody|1ST0000-10|Lyophilized recombinant human IL-3 standard|10ng/tubex2AR1107|Biotinylated anti- human IL-3 antibody|130ul(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex(ABC)|130ul(dilution 1:100)AR1106-1|Sample diluent buffer|30mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Menge | 1x96 Wells |
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Hersteller | Boster |
Herstellerland | USA |
Sensitivity | <1pg/ml |
Specific Against | Human |
Antigen Name | Interleukin-3 (IL3) |
Detection Range | 15.6pg/ml-1000pg/ml |
Alias | Burst promoting activity, Colony stimulating factor multiple, Eosinophil colony stimulating factor, Hematopoietic growth factor, IL 3, IL-3, IL3, IL3_HUMAN, Interleukin 3, Interleukin-3, Mast cell growth factor, MCGF, Multi CSF, Multilineag |