BioAim Scientific (Kanada)
The Bioaim Human IL-27 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of IL-27 in Human serum, plasma and cell culture media. An antibody specific for Human IL-27 was coated on a 96-well plate. Standards and samples are added to the wells and any IL-27 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human IL-27 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of IL-27 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Inflammation;
Interleukin-27(IL-27) Background: Interleukin 27 (IL27) is a heterodimeric cytokine belonging to the IL-12 family that is composed of two subunits; Epstein-Barr virus (EBV)-induced gene 3 (EBI3) (also known as IL-27B) and IL27-p28 (known as IL-30). IL-27 is produced by antigen-presenting cells. IL-27 plays an important function in regulating the activity of B- and T-lymphocytes. The effects of IL-27 are eliciting by its interaction with a specific cell-surface receptor complex composed of two proteins known as IL27R and gp130. IL-27 has both anti- and pro-inflammatory properties. As an anti-inflammatory, IL-27 seems to induce a general negative feedback program that limits T and NK-T cell activity. At the onset of infection, IL-27 induces an IL-12 receptor on naïve CD4+ T cells, making them susceptible to subsequent IL-12 activity (and possible Th1 development).
IL-27, IL-27AA, IL27p28, IL30, p28, IL27
Serum, plasma, Cell culture supernatant
|Detection Range||81.9-20000 pg/ml|
|Alias||IL-27, IL-27AA, IL27p28, IL30, p28, IL27|