Human IL-2 R beta ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human IL-2 R beta ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of IL-2 R beta in human serum and plasma. An antibody specific for human IL-2 R beta was coated on a 96-well plate. Standards and samples are added to the wells and any IL-2 R beta present binds to the immobilized antibody. The wells are washed and biotinylated anti-human IL-2 R beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of IL-2 R beta present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Hematologic Cancer;

specific against

Human

detection target

interleukin 2 receptor subunit beta( IL-2 R β )
Background: The interleukin 2 receptor, which is involved in T cell-mediated immune responses, is present in 3 forms with respect to ability to bind interlukin 2. The low affinity form is a monomer of the alpha subunit and is not involved in signal transduction. The intermediate affinity form consists of an alpha/beta subunit heterodimer, while the high affinity form consists of an alpha/beta/gamma subunit heterotrimer. Both the intermediate and high affinity forms of the receptor are involved in receptor-mediated endocytosis and transduction of mitogenic signals from interleukin 2. The protein encoded by this gene represents the beta subunit and is a type I membrane protein.

alias

CD122, IL15RB, P70-75

sensitivity

10 pg/ml

detection range

24.6-6000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P14784