Human IL-1F9 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human IL-1F9 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of IL-1F9 in Human serum, plasma and cell culture media. An antibody specific for Human IL-1F9 was coated on a 96-well plate. Standards and samples are added to the wells and any IL-1F9 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human IL-1F9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of IL-1F9 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Inflammation;

specific against

Human

detection target

interleukin 36, gamma (IL-1F9)
Background: IL-36 gamma ,IL-1F9 is a member of the IL-1 family. Highest levels of IL‑36 gamma are produced by Langerhans cells, keratinocytes, and stomach Chief cells and parietal cells; these cells contribute to first‑line defense against pathogens in the skin, lungs and digestive tract. Its expression is induced by LPS treatment of monocytes, and by IL‑ alpha / beta, IL‑17 or TNF‑ alpha treatment of keratinocytes and bronchial epithelia.

alias

IL-1F9, IL-1H1, IL-1RP2, IL1E, IL1F9, IL1H1, IL1RP2

sensitivity

8 pg/ml

detection range

12.3-3000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

Q9NZH8