Human IL-18BP PicoKine ELISA Kit

Brand

Boster

short description

For quantitative detection of human IL-18BP in cell culture supernates, serum, plasma(heparin, EDTA) and urine.

spezie

Human

Cross Reaction

There is no detectable cross-reactivity with other relevant proteins.

detection target

human IL-18BP
Protein name: Interleukin-18-binding protein
Gene Full Name: interleukin 18 binding protein
abbreviation: IL18BP
Background: Interleukin-18-binding protein, also known as IL18BPa, is a protein that in humans is encoded by the IL18BP gene. It is mapped to 11q13.4. The protein encoded by this gene is an inhibitor of the proinflammatory cytokine IL18. This protein binds to IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production. This protein is constitutively expressed and secreted in mononuclear cells. The expression of this protein can be enhanced by IFN-gamma. It has been found that IL18BP expression in intestinal tissue is increased in endothelial cells as well as cells of the submucosa and overlying lymphoid aggregates in Crohn disease.
ID:O95998
ID:10068

alias

I18BP_HUMAN|IL-18BP|IL18BP|Interleukin-18-binding protein|Tadekinig-alfa

Origin

Nativ and recombinant human IL-18BP

Antibodies

a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat

Immunogene

NSO, T31-G194

sensitivity

<10pg/ml

detection range

93.7pg/ml-6000pg/ml

pack size

1x96 Wells

Reproducibility

|Intra-Assay Precision|Inter-Assay Precision
Sample|1|2|3|1|2|3
n|16|16|16|24|24|24
Mean(pg/ml)|1289|3561|5320|999|2895|4672
Standard deviation|61.9|203|228.76|51.95|189|262
CV(%)|4.8|5.7|4.3|5.2|6.5|5.6

TMB(tetramethylbenzidine) incubation time

20-25

storage and Stability

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Principle of the Elisa

Boster′s human IL-18BP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-18BP has been precoated onto 96-well plates. Standards(NSO, T31-G194) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-18BP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-18BP amount of sample captured in plate.

Assay Procedure Step 1

Aliquot 0.1ml per well of the 6000pg/ml, 3000pg/ml, 1500pg/ml, 750pg/ml, 375pg/ml, 187.5pg/ml, 93.7pg/m human IL-18BP standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or urine to each empty well. See ﾓSample Dilution Guidelineﾔ above for details. It is recommended that each human IL-18BP standard solution and each sample be measured in duplicate.

tipical result

Concentration(pg/ml)|0|93.7|187.5|375|750|1500|3000|6000 O.D.|0.060|0.095|0.152|0.275|0.497|0.948|1.596|2.309

Kit Content

Catalog number|Description|Quantity
-|96-well plate precoated with anti- human IL-18BP antibody|1
ST0000-10|Lyophilized recombinant human IL-18BP standard|10ng/tube�2
AR1107|Biotinylated anti- human IL-18BP antibody|130µl(dilution 1:100)
AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)
AR1106-1|Sample diluent buffer|30ml
AR1106-2|Antibody diluent buffer|12ml
AR1106-3|ABC diluent buffer|12ml
AR1104|TMB color developing agent|10ml
AR1105|TMB stop solution|10ml
-|Adhesive cover|4