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Boster
For quantitative detection of human IL-17E in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Human
There is no detectable cross-reactivity with other relevant proteins.
Human IL-17EProtein name: Interleukin-25Gene Full Name: interleukin 25abbreviation: IL25Background: Interleukin-25 (IL-25), also known as interleukin-17E (IL-17E), is a protein that in humans is encoded by the IL25 gene. It is a cytokine that belongs to the IL-17 cytokine family and mapped to 14q11.2. IL-25 is secreted by type 2 helper T cells (Th2) and mast cells. It has been found that IL-25 can induce Th2-type cytokine expression and production by non-T, non-B accessory cells that express high levels of major histocompatibility complex class II and low levels of Cd11c. This cytokine also can induce NF-?B activation, and stimulate the production of IL8. What′s more, IL-25 is an important molecule controlling immunity of the gut and has been implicated in chronic inflammation associated with the gastrointestinal tract. ID:Q9H293 ID:64806
IL 17E|IL 25|IL-17E|IL-25|IL17e|IL25|IL25_HUMAN|Interleukin 17E|Interleukin 25|Interleukin-17E|Interleukin-25|Interleukin17E|Interleukin25|OTTHUMP00000027946|OTTHUMP00000246238|UNQ3120/PRO10272
Nativ and recombinant human IL-17E
a monoclonal antibody from mouse|a biotinylated polyclonal antibody from goat
E.coli, Y33-G177
<10pg/ml
62.5pg/ml-4000pg/ml
1x96 Wells
|Intra-Assay Precision|Inter-Assay Precision Sample|1|2|3|1|2|3 n|16|16|16|24|24|24 Mean(pg/ml)|621|1969|2795|730|2103|3065 Standard deviation|24.2|90.6|150.9|30.7|119.9|208.4 CV(%)|3.9|4.6|5.4|4.2|5.7|6.8
20-25
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Boster′s human IL-17E ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IL-17E has been precoated onto 96-well plates. Standards(E.coli, Y33-G177) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IL-17E is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IL-17E amount of sample captured in plate.
Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human IL-17E standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See モSample Dilution Guidelineヤ above for details. We recommend that each human IL-17E standard solution and each sample is measured in duplicate.
Concentration(pg/ml)|0|62.5|125|250|500|1000|2000|4000 O.D.|0.055|0.093|0.153|0.248|0.44|0.821|1.349|2.075
Catalog number|Description|Quantity-|96-well plate precoated with anti- human IL-17E antibody|1ST0000-10|Lyophilized recombinant human IL-17E standard|10ng/tube�2AR1107|Biotinylated anti- human IL-17E antibody|130µl(dilution 1:100)AR1103|Avidin-Biotin-Peroxidase Complex (ABC)|130µl(dilution 1:100)AR1106-1|Sample diluent buffer|30 mlAR1106-2|Antibody diluent buffer|12mlAR1106-3|ABC diluent buffer|12mlAR1104|TMB color developing agent|10mlAR1105|TMB stop solution|10ml-|Adhesive cover|4
Menge | 1x96 Wells |
---|---|
Hersteller | Boster |
Herstellerland | USA |
Sensitivity | <10pg/ml |
Specific Against | Human |
Antigen Name | Interleukin-25 (IL25) |
Detection Range | 62.5pg/ml-4000pg/ml |
Alias | IL 17E, IL 25, IL-17E, IL-25, IL17e, IL25, IL25_HUMAN, Interleukin 17E, Interleukin 25, Interleukin-17E, Interleukin-25, Interleukin17E, Interleukin25, OTTHUMP00000027946, OTTHUMP00000246238, UNQ3120/PRO10272 |