BioAim Scientific (Kanada)
The Bioaim Human IL-16 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of IL-16 in Human serum, plasma and cell culture media. An antibody specific for Human IL-16 was coated on a 96-well plate. Standards and samples are added to the wells and any IL-16 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human IL-16 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of IL-16 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Inflammation;
Interleukin-16(IL-16) Background: Interleukin 16 (IL-16), also named lymphocyte chemoattractant factor (LCF), is a 14-17 kDa single chain non-glycosylated polypeptide. The protein encoded by this gene is a pleiotropic cytokine that functions as a chemoattractant, a modulator of T cell activation, and an inhibitor of HIV replication. The signaling process of this cytokine is mediated by CD4. The product of this gene undergoes proteolytic processing, which is found to yield two functional proteins. The cytokine function is exclusively attributed to the secreted C-terminal peptide, while the N-terminal product may play a role in cell cycle control.
LCF, NIL16, PRIL16, prIL-16
Serum, plasma, Cell culture supernatant
|Detection Range||4.1-3000 pg/ml|
|Alias||LCF, NIL16, PRIL16, prIL-16|