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Human IL-15 / IL15 / Interleukin 15 ELISA Pair Set

Product Code: SEK10360

Verfügbarkeit: Auf Lager

Human IL-15 / IL15 / Interleukin 15 ELISA Pair Set

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Beschreibung

Details

Human IL-15 / IL15 / Interleukin 15 ELISA Pair Set

Brand

Sino Biological

short description

The Human IL-15 / IL15 / Interleukin 15 ELISA Pair Set is for the quantitative determination of Human IL-15 / IL15 / Interleukin 15. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human IL-15 / IL15 / Interleukin 15 coated on a 96-well plate. Standards and samples are added to the wells, and any Human IL-15 / IL15 / Interleukin 15 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Human IL-15 / IL15 / Interleukin 15 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human IL-15 / IL15 / Interleukin 15 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

spezie

Human

detection target

IL15
Standard (antigene): Each vial contains 28 ng of recombinant Human IL-15 / IL15 / Interleukin 15. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 600 pg/mL is recommended.
capture antibody type: 1 mg/mL of rabbit anti-Human IL-15 / IL15 / Interleukin 15 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 µg/mL in PBS before coating. (Catalog: # 10360-R112)
detection antibody type: 0.2 mg/mL of rabbit anti-Human IL-15 / IL15 / Interleukin 15 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4°C;). Dilute to working concentration of 0.25 µg/mL in detection antibody dilution buffer before use. (Catalog: # 10360-R111)
Lable: HRP

sensitivity

The minimum detectable dose of Human IL-15 / IL15 / Interleukin 15 was determined to be approximately 9.38 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

Detection limitation

9.38 pg/ml

certificate

ISO9001-2008

pack size

5 and 15 Plates

storage and Stability

Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Store at 4°C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.

Weitere Informationen

Weitere Informationen

Hersteller Sino Biological
Herstellerland China
Sensitivity The minimum detectable dose of Human IL-15 / IL15 / Interleukin 15 was determined to be approximately 9.38 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero stan
Specific Against Human
Antigen Name IL-15
Detection Range 9.38 pg/ml
Zertifikat ISO9001-2008
Alias IL-15 / IL15 / Interleukin 15
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Datasheet