Human IL-12 p70 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human IL-12p70 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of IL-12p70 in Human serum, plasma and cell culture media. An antibody specific for Human IL-12p70 was coated on a 96-well plate. Standards and samples are added to the wells and any IL-12p70 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human IL-12p70 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of IL-12p70 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Inflammation;

specific against

Human

detection target

interleukin 12A(IL-12 p70)
Background: Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells. This cytokine is required for the T-cell-independent induction of interferon gamma (INF-β), and is important for the differentiation of both Th1 and Th2 cells. The responses of lymphocytes to this cytokine are mediated by the activator of transcription protein STAT4.

alias

CLMF, IL12A, NFSK, NKSF1, P35

sensitivity

< 1 pg/ml

detection range

0.82-600 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P29459