For the quantitative determination of Human IL-12 (IL12A & IL12B Heterodimer) concentration in cell culture supernates.
Human peripheral blood mononuclear cells ?2 x 10E6 cells/mL? were cultured in DMEM supplemented with 5% bovine calf serum, 50 µM ?-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, and 100 µg/mL streptomycin sulfate, stimulated with 10 µg/mL PHA for 1 day. Aliquots of the cell culture supernates were removed and assayed for levels of natural IL12A+IL12B, and measured 0.34 ng/mL.
This assay recognizes recombinant Human IL12A-his+IL12B. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
IL12A & IL12BAntigene: CT011-H08Hcapture antibody type: rabbit mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Antigen Name||IL-12 (IL12A & IL12B Heterodimer)|
|Detection Range||39.07-2500 pg/ml|
|Alias||IL-12 (IL12A & IL12B Heterodimer)|