Human IL-1 R4 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human IL-1R4 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of IL-1R4 in human serum and plasma. An antibody specific for human IL-1R4 was coated on a 96-well plate. Standards and samples are added to the wells and any IL-1R4 present binds to the immobilized antibody. The wells are washed and biotinylated anti-human IL-1R4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of IL-1R4 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area inflammation;

specific against

Human

detection target

interleukin 1 receptor-like 1( IL-1 R4 )
Background: IL-1 R4, also known as ST2, is a component of the receptor for the proinflammatory cytokine Interleukin-33. The IL-33 receptor additionally includes IL-1 RAcp which also associates with IL-1 RI and IL-1 R rp2/IL-1 R6. A soluble form of ST2 functions as a decoy receptor. IL-33 promotes Th2-biased immune responses, resulting in eosinophilia, mast cell activation, and allergic inflammation. In addition, IL-33 enhances inflammation-associated hypernociception and protects from atherosclerosis and cardiac myocyte hypertrophy. Soluble ST2 is elevated in the serum under inflammatory conditions including allergic asthma, sepsis, trauma, dengue fever, pulmonary disease, and lupus. It is also associated aortic stenosis, congestive cardiomyopathy, and risk of cardiovascular heart failure and death.

alias

IL33R, DER4, ST2V, T1, FIT-1, ST2, ST2L

sensitivity

< 1 pg/ml

detection range

1.6-1200 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

Q01638