The Human IkB alpha / NFKBIA ELISA Pair Set is for the quantitative determination of Human IkB alpha / NFKBIA. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IkB alpha / NFKBIA coated on a 96-well plate. Standards and samples are added to the wells, and any IkB alpha / NFKBIA present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-IkB alpha / NFKBIA monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of IkB alpha / NFKBIA present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
NFKBIAStandard (antigene): Each vial contains 22 ng of recombinant NFKBIA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.8 ng/mL is recommended. capture antibody type: 1.0 mg/mL of rabbit anti-NFKBIA monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 µg/mL in CBS before coating. (Catalog: # 12045-R056)detection antibody type: 0.5 mg/mL mouse anti-NFKBIA monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4). Dilute to working concentration of 0.5 µg/mL in detection antibody dilution buffer before use. (Catalog: # 12045-MM02)Lable: HRP
The minimum detectable dose of Human IkB alpha / NFKBIA was determined to be approximately 12.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
5 and 15 Plates
Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
|Sensitivity||The minimum detectable dose of Human IkB alpha / NFKBIA was determined to be approximately 12.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.|
|Antigen Name||IkB alpha|
|Detection Range||12.5 pg/ml|
|Alias||IkB alpha / NFKBIA|