For the quantitative determination of Human IkB alpha / NFKBIA concentration in cell lysate.
The concentration of Human NFKBIA detected in Hela, HepG2 and Jurkat cell lysates were respectively 156.17 ng/mL, 146.00 ng/mL and 53.75 ng/mL.The total protein concentration of them were about 9.3 mg/mL, 8.5 mg/mL and 6mg/mL.
This assay recognizes both recombinant and natural Human NFKBIA. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
NFKBIAAntigene: 12045-H07Ecapture antibody type: rabbit mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Antigen Name||IkB alpha|
|Detection Range||12.5-800 pg/ml|
|Alias||IkB alpha / NFKBIA|