Profile Informationen
Login Daten
oder Einloggen
BioAim Scientific (Kanada)
The Bioaim Human IgG ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of IgG in human serum and plasma. An antibody specific for human IgG was coated on a 96-well plate. Standards and samples are added to the wells and any IgG present binds to the immobilized antibody. The wells are washed and biotinylated anti-human IgG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of IgG present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Immune response;
Human
IgG( IgG ) Background:
20 pg/ml
20.6-15000 pg/ml
Serum, plasma, Cell culture supernatant
1x96 Wells
4.5 h
Sandwich ELISA
Menge | 1x96 Wells |
---|---|
Hersteller | BioAim Scientific |
Herstellerland | Kanada |
Sensitivity | 20 pg/ml |
Specific Against | Human |
Antigen Name | IgG |
Detection Range | 20.6-15000 pg/ml |