For the quantitative determination of Human IGFBP3 concentration in serum.
The average concentration of Human IGFBP3 detected in 10 human serum from apparently healthy people is 2220 +/- 293 ng/mL ranging from 1579 to 2489 ng/mL.
This assay recognizes both recombinant and natural Human IGFBP3. The factors listed below were prepared at 250 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
IGFBP3Antigene: 10430-H07Hcapture antibody type: rabbit mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Detection Range||12.5-800 pg/ml|