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Human IGF1R / CD221 / CD221 ELISA Pair Set

Product Code: SEK10164

Verfügbarkeit: Auf Lager

Human IGF1R / CD221 / CD221 ELISA Pair Set

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Beschreibung

Details

Human IGF1R / CD221 / CD221 ELISA Pair Set

Brand

Sino Biological

short description

The human IGF1R / CD221 ELISA Pair Set is for the quantitative determination of human IGF1R / CD221. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IGF1R / CD221 coated on a 96-well plate. Standards and samples are added to the wells, and any IGF1R / CD221 present binds to the immobilized antibody. The wells are washed and a biotinylated rabbit anti-IGF1R / CD221 polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". To produces color in proportion to the amount of IGF1R / CD221 present in the sample strepavidin-HRP and TMB substrate solution are loaded. The absorbances of the microwell are read at 450 nm.

spezie

Human

detection target

IGF1R
Standard (antigene): Each vial contains 140 ng of recombinant IGF1R. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 10 ng/mL is recommended.
capture antibody type: 0.5 mg/mL of mouse anti-IGF1R monoclonal antibody. Dilute to a working concentration of 1 µg/mL in CBS before coating. (Catalog: # 10164-MM07)
detection antibody type: Each vial contains 35 µg biotinylated rabbit anti-IGF1R polyclonal antibody. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -70°C; in a manual defrost freezer. Dilute to a working concentration of 0.25 µg/mL in detection antibody dilution buffer before use.
Lable: Biotin

sensitivity

The minimum detectable dose of human IGF1R / CD221 was determined to be approximately 156 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

Detection limitation

156 pg/ml

certificate

ISO9001-2008

pack size

5 and 15 Plates

storage and Stability

Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles. Streptavidin-HRP: Store at 4°C and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.

Weitere Informationen

Weitere Informationen

Hersteller Sino Biological
Herstellerland China
Sensitivity The minimum detectable dose of human IGF1R / CD221 was determined to be approximately 156 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Specific Against Human
Antigen Name IGF1R
Detection Range 156 pg/ml
Zertifikat ISO9001-2008
Alias IGF1R / CD221 / CD221
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