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Sino Biological
The human IGF1R / CD221 ELISA Pair Set is for the quantitative determination of human IGF1R / CD221. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IGF1R / CD221 coated on a 96-well plate. Standards and samples are added to the wells, and any IGF1R / CD221 present binds to the immobilized antibody. The wells are washed and a biotinylated rabbit anti-IGF1R / CD221 polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". To produces color in proportion to the amount of IGF1R / CD221 present in the sample strepavidin-HRP and TMB substrate solution are loaded. The absorbances of the microwell are read at 450 nm.
Human
IGF1RStandard (antigene): Each vial contains 140 ng of recombinant IGF1R. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 10 ng/mL is recommended. capture antibody type: 0.5 mg/mL of mouse anti-IGF1R monoclonal antibody. Dilute to a working concentration of 1 µg/mL in CBS before coating. (Catalog: # 10164-MM07)detection antibody type: Each vial contains 35 µg biotinylated rabbit anti-IGF1R polyclonal antibody. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -70°C; in a manual defrost freezer. Dilute to a working concentration of 0.25 µg/mL in detection antibody dilution buffer before use.Lable: Biotin
The minimum detectable dose of human IGF1R / CD221 was determined to be approximately 156 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
156 pg/ml
ISO9001-2008
5 and 15 Plates
Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles. Streptavidin-HRP: Store at 4°C and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.
Hersteller | Sino Biological |
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Herstellerland | China |
Sensitivity | The minimum detectable dose of human IGF1R / CD221 was determined to be approximately 156 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard. |
Specific Against | Human |
Antigen Name | IGF1R |
Detection Range | 156 pg/ml |
Zertifikat | ISO9001-2008 |
Alias | IGF1R / CD221 / CD221 |