Human I-TAC ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human I-TAC ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of I-TAC in Human serum, plasma and cell culture media. An antibody specific for Human I-TAC was coated on a 96-well plate. Standards and samples are added to the wells and any I-TAC present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human I-TAC antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of I-TAC present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis; inflammation;

specific against

Human

detection target

C-X-C motif chemokine 11(I-TAC)
Background: Interferon-inducible T cell a chemoattractant (I-TAC), also known as SCYB9B, H174 and beta-R1, is a non-ELR motif-containing CXC chemokine. I-TAC shares 36% and 37% amino acid sequence homology with IP-10 and MIG, respectively. I-TAC is expressed at low levels in normal tissues, including thymus, spleen and pancreas. Gene expression of CXCL11 is strongly induced by IFN-β and IFN-β, and weakly induced by IFN-α. This chemokine elicits its effects on its target cells by interacting with the cell surface chemokine receptor CXCR3, with a higher affinity than do the other ligands for this receptor, CXCL9 and CXCL10. CXCL11 is chemotactic for activated T cells.

alias

H174, I-TAC, IP-9, IP9, SCYB11, SCYB9B, b-R1

sensitivity

< 1 pg/ml

detection range

2.5-600 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

O14625