BioAim Scientific (Kanada)
The Bioaim Human I-309 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of I-309 in Human serum, plasma and cell culture media. An antibody specific for Human I-309 was coated on a 96-well plate. Standards and samples are added to the wells and any I-309 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human I-309 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of I-309 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Angiogenesis; inflammation;
C-C motif chemokine ligand 1(I-309) Background: I-309, a member of the CC subfamily of chemokines, was initially identified by subtractive hybridization as a transcript that was present in a gamma/delta T cell line, but not in EBV-transformed B cells. Human I-309 is assumed to be a homolog of the mouse TCA-3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8.
I-309, P500, SCYA1, SISe, TCA3
Serum, plasma, Cell culture supernatant
|Antigen Name||C-C motif chemokine ligand 1|
|Detection Range||4.1-3000 pg/ml|
|Alias||I-309, P500, SCYA1, SISe, TCA3|