For the quantitative determination of Human HSPB1 concentration in serum.
The average concentration of Human HSP27 detected in 10 human serum from apparently healthy people is 19.7 +/- 8.5ng/mL ranging from 9.7 to 31.7 ng/mL.
This assay recognizes both recombinant and natural Human HSP27. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
HSPB1Antigene: 10351-H08Ecapture antibody type: rabbit mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Detection Range||9.38-600 pg/ml|