Human Hemopexin / HPX ELISA Pair Set

Brand

Sino Biological

short description

The Human Hemopexin / HPX ELISA Pair Set is for the quantitative determination of Human Hemopexin / HPX. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human Hemopexin / HPX coated on a 96-well plate. Standards and samples are added to the wells, and any Human Hemopexin / HPX present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-Human Hemopexin / HPX polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human Hemopexin / HPX present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

spezie

Human

detection target

HPX
Standard (antigene): Each vial contains 48 ng of recombinant Human Hemopexin / HPX. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1200 pg/mL is recommended.
capture antibody type: 1 mg/mL of mouse anti-Human Hemopexin / HPX monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 µg/mL in PBS before coating. (Catalog: # 10870-MM08)
detection antibody type: 0.4 mg/mL of rabbit anti-Human Hemopexin / HPX polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4). Dilute to working concentration of 0.5 µg/mL in detection antibody dilution buffer before use.
Lable: HRP

sensitivity

The minimum detectable dose of Human Hemopexin / HPX was determined to be approximately 18.8 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

Detection limitation

18.8 pg/ml

certificate

ISO9001-2008

pack size

5 and 15 Plates

storage and Stability

Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.