Human HCC-1 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human HCC-1 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of HCC-1 in Human serum, plasma and cell culture media. An antibody specific for Human HCC-1 was coated on a 96-well plate. Standards and samples are added to the wells and any HCC-1 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human HCC-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of HCC-1 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis; inflammation;

specific against

Human

detection target

SAP domain containing ribonucleoprotein (HCC-1)
Background: CCL14, is a member of the chemokine superfamily of ligands. In-depth genome analysis has determined that there is no mouse ortholog for human CCL14. Two alternatively spliced isoforms of CCL14, CCL14a/HCC-1 and CCL14b/HHC-3, have been identified. CCL14a/HCC-1 is cleaved into a 74 aa secreted bioactive peptide by Plasmin or u-Plasminogen Activator/Urokinase. CCL14a/HCC-1 binds and activates CCR1, CCR3, and CCR5; it also binds the non-signaling chemokine decoy receptors DARC and D6. CCL14a/HCC-1 is widely expressed in normal tissues including spleen, liver, skeletal and heart muscle, gut, and bone marrow, and it circulates at high concentrations in the plasma.

alias

CIP29, HCC1, HSPC316, THO1

sensitivity

3 pg/ml

detection range

6.1-1500 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

Q14498