For the quantitative determination of Human Granulin / Progranulin / GRN concentration in serum.
The average concentration of GRN in 10 normal human serum is 60.44 +/- 11.05 ng/mL ranging from 41.37 to 79.70 ng/mL.
This assay recognizes both recombinant and natural Human GRN. Mouse GRN was prepared at 200 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
GRNAntigene: 10826-H08Hcapture antibody type: mouse mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Detection Range||78.13-5000 pg/ml|
|Alias||Granulin / Progranulin / GRN|