BioAim Scientific (Kanada)
The Bioaim Human GM-CSF ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of GM-CSF in human serum and plasma. An antibody specific for human GM-CSF was coated on a 96-well plate. Standards and samples are added to the wells and any GM-CSF present binds to the immobilized antibody. The wells are washed and biotinylated anti-human GM-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of GM-CSF present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Inflammation;
granulocyte-macrophage colony-stimulating factor ( GM-CSF ) Background: GM-CSF, also known as colony stimulating factor 2 (CSF2), is a monomeric glycoprotein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts that functions as a cytokine. The pharmaceutical analogs of naturally occurring GM-CSF are called sargramostim and molgramostim. GM-CSF induces the development of monocytes, neutrophils, eosinophils, and myeloid and dermal dendritic cells. It also acts as a neutrophil and dendritic cell chemoattractant. GM-CSF promotes Th1 and Th17 cell mediated autoimmune inflammation as well as the inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it cooperates with G-CSF in promoting tumor cell proliferation and invasion. GM-CSF signals through a receptor complex composed of GM-CSF R alpha and the Common beta Chain (beta c) with Syndecan-2 as a potential co-receptor.
Serum, plasma, Cell culture supernatant
|Antigen Name||granulocyte-macrophage colony-stimulating factor|
|Detection Range||2.05-500 pg/ml|