Human GLP-1 EasyTestTM competitive ELISA kit

Brand

BioAim Scientific (Kanada)

short description

The BioAim human GLP-1 EasyTestTM Competitive ELISA kit can quantitatively measure GLP-1 in human serum or plasma. Other matrices, such as urine and tissue, may be suitable but have not been validated. It is a simple and rapid technology for the quantitation of antigen in a range of sample matrices. The whole process takes 2 hours with high accuracy and precision.
Research Area Peptide hormone;

specific against

Human

detection target

glucagon(GLP-1)
Background: GLP-1 secretion by ileal L cells is dependent on the presence of nutrients in the lumen of the small intestine. The secretagogues (agents that cause or stimulate secretion) of this hormone include major nutrients like carbohydrate, protein and lipid. Once in the circulation, GLP-1 has a half-life of less than 2 minutes, due to rapid degradation by the enzyme dipeptidyl peptidase-4. It is a potent antihyperglycemic hormone, inducing glucose-dependent stimulation of insulin secretion while suppressing glucagon secretion. Such glucose-dependent action is particularly attractive because, when the plasma glucose concentration is in the normal fasting range, GLP-1 no longer stimulates insulin to cause hypoglycemia. GLP-1 appears to restore the glucose sensitivity of pancreatic β-cells, with the mechanism possibly involving the increased expression of GLUT2 and glucokinase. GLP-1 is also known to inhibit pancreatic β-cell apoptosis and stimulate the proliferation and differentiation of insulin-secreting β-cells. In addition, GLP-1 inhibits gastric secretion and motility. This delays and protracts carbohydrate absorption and contributes to a satiating effect.

alias

GLP1, GLP2, GRPP

sensitivity

90 pg/ml

detection range

24.4-100000 pg/ml

sample type

Serum, plasma

pack size

1x96 Wells

Assay time

2.0 h

Principle of the Elisa

EIA

SwissProtID

P01275