BioAim Scientific (Kanada)
The Bioaim Human GITR ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of GITR in human serum and plasma. An antibody specific for human GITR was coated on a 96-well plate. Standards and samples are added to the wells and any GITR present binds to the immobilized antibody. The wells are washed and biotinylated anti-human GITR antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of GITR present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area T Cell stimulation;
TNF receptor superfamily member 18 ( GITR ) Background: GITR (glucocorticoid-induced tumor necrosis factor receptor), also known as AITR and TNFRSF18, is a transmembrane TNF receptor superfamily member that functions in immune regulation. GITR is expressed on CD4+CD25+ regulatory T cells (Treg) as well as on subsets of thymocytes, lymph node cells, and splenocytes, and it is upregulated on antigen-activated conventional CD4+ and CD8+ T cells. GITR binding by GITR Ligand/TNFSF18 costimulates the proliferation and activation of CD4+ or CD8+ conventional T cells. It also induces the proliferation of Treg but inhibits the ability of Treg to suppress immune responses. This can result in the development of autoimmunity, increased tumor cell killing by effector T cells, and increased inflammation in arthritis, allergic asthma, and inflammatory bowel disease. GITR is also expressed on sympathetic neurons where it enhances NGF-induced neurite outgrowth and branching.
AITR, CD357, GITR,GITR-D
Serum, plasma, Cell culture supernatant
|Antigen Name||TNF receptor superfamily member 18|
|Detection Range||13.7-10000 pg/ml|
|Alias||AITR, CD357, GITR,GITR-D|