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BioAim Scientific (Kanada)
The Bioaim Human GH ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of GH in human serum and plasma. An antibody specific for human GH was coated on a 96-well plate. Standards and samples are added to the wells and any GH present binds to the immobilized antibody. The wells are washed and biotinylated anti-human GH antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of GH present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Aging; peptide hormone;
Human
growth hormone( GH ) Background: Growth hormone (GH), also known as somatotropin (or as human growth hormone [hGH or HGH] in its human form), is apeptide hormone that stimulates growth, cell reproduction, and cell regeneration in humans and other animals. It is thus important inhuman development. It is a type of mitogen which is specific only to certain kinds of cells. Growth hormone is a 191-amino acid, single-chain polypeptide that is synthesized, stored, and secreted by somatotropic cells within the lateral wings of the anterior pituitary gland. GH is a stress hormone that raises the concentration of glucose and free fatty acids. It also stimulates production of IGF-1.
GH, GH-N, GHB5, GHN, IGHD1B, hGH-N
<1 pg/ml
2.5-600 pg/ml
Serum, plasma, Cell culture supernatant
1x96 Wells
4.5 h
Sandwich ELISA
P01241
Menge | 1x96 Wells |
---|---|
Hersteller | BioAim Scientific |
Herstellerland | Kanada |
Sensitivity | <1 pg/ml |
Specific Against | Human |
Antigen Name | growth hormone |
Detection Range | 2.5-600 pg/ml |
Alias | GH, GH-N, GHB5, GHN, IGHD1B, hGH-N |