Human Furin ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human Furin ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Furin in human serum and plasma. An antibody specific for human Furin was coated on a 96-well plate. Standards and samples are added to the wells and any Furin present binds to the immobilized antibody. The wells are washed and biotinylated anti-human Furin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Furin present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis, cell survival, and immune response;

specific against

Human

detection target

Furin ( Furin )
Background: Furin is also known as PACE (Paired basic Amino acid Cleaving Enzyme). This family of serine proteases consists of furin and proprotein convertases encoded by PCSK genes. They are important in a variety of processes. Furin has an essential role in embryogenesis and homeostasis and is implicated in various pathologies such as cancer, neurodegenerative diseases and anthrax. Mutations in the PC1/PCSK1 gene are associated with obesity and impaired prohormone processing.

alias

FUR, PACE, PCSK3, SPC1

sensitivity

8 pg/ml

detection range

13.7-10000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P09958