Human Flt-3L ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human Flt-3L ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Flt-3L in human serum and plasma. An antibody specific for human Flt-3L was coated on a 96-well plate. Standards and samples are added to the wells and any Flt-3L present binds to the immobilized antibody. The wells are washed and biotinylated anti-human Flt-3L antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Flt-3L present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Cell survival; proliferation; motility;

specific against

Human

detection target

fms related tyrosine kinase 3 ligand ( Flt-3L )
Background: Flt-3 Ligand is an alpha-helical cytokine that promotes the differentiation of multiple hematopoietic cell lineages. Flt-3 Ligand is expressed as a noncovalently-linked dimer by T cells and bone marrow and thymic fibroblasts. It is an endogenous small molecule that functions as a cytokine and growth factor that increases the number of immune cells (lymphocytes (B cells and T cells)) by activating the hematopoietic progenitors. It acts by binding to and activating FLT3 (CD135) which is found on what (in mice) are called multipotent progenitor (MPP) and common lymphoid progenitor (CLP) cells. It also induces the mobilization of the hematopoietic progenitors and stem cells in vivo which may help the system to kill cancer cells. FLT3L is crucial for steady-state plasmacytoid dendritic cell (pDC) and classical dendritic cell (cDC) development. A lack of FLT3L results in low levels of dendritic cells.

alias

FL, FLT3L

sensitivity

< 1 pg/ml

detection range

1.6-400 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P49771