Human FGF-6 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human FGF-6 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of FGF-6 in Human serum, plasma and cell culture media. An antibody specific for Human FGF-6 was coated on a 96-well plate. Standards and samples are added to the wells and any FGF-6 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human FGF-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of FGF-6 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis; cell growth;

specific against

Human

detection target

Fibroblast growth factor 6(FGF-6)
Background: Fibroblast Growth Factor-6 (FGF-6), also known as HST-2, is a 25-28 kDa member of the FGF family of heparin binding polypeptides which are potent regulators of cell proliferation, differentiation, and function. FGF proteins contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure. It binds and signals primarily through FGF R1c, 2c, and 4. FGF-6 functions as a mitogen for fibroblasts, vascular endothelial cells, and prostate carcinoma cells, and N-linked glycosylation is required for the full mitogenic effect. FGF-6 expression is restricted to skeletal muscle during development although it can be upregulated in prostate cancer and Kaposi sarcoma. In the adult, FGF-6 is upregulated in injured skeletal muscle and is required for muscle regeneration. FGF-6 inhibits the terminal differentiation of myoblasts and also cooperates with TGF-beta 2 to promote chondrogenesis in embryonic somites.

alias

HBGF-6, HST2

sensitivity

4 pg/ml

detection range

8.2-6000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P10767