Human FGF-4 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human FGF4 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of FGF4 in human serum and plasma. An antibody specific for human FGF4 was coated on a 96-well plate. Standards and samples are added to the wells and any FGF4 present binds to the immobilized antibody. The wells are washed and biotinylated anti-human FGF4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of FGF4 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis; cell growth;

specific against

Human

detection target

fibroblast growth factor 4( FGF-4 )
Background: FGF-4 (Fibroblast Growth Factor-4), also known as FGF-K and K-FGF, is a secreted angiogenic factor that is mitogenic for fibroblasts and endothelial cells. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. During development, it is required for maintenance of the trophoectoderm and primitive endoderm, and it cooperates with FGF-8 in the apical ectodermal ridge. FGF-4 promotes embryonic stem cell proliferation and may also aid stem cell differentiation.

alias

HBGF-4, HST, HST-1, HSTF1, K-FGF, KFGF

sensitivity

15 pg/ml

detection range

24.7-18000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P08620