BioAim Scientific (Kanada)
The Bioaim Human FGF-21 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of FGF-21 in Human serum, plasma and cell culture media. An antibody specific for Human FGF-21 was coated on a 96-well plate. Standards and samples are added to the wells and any FGF-21 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human FGF-21 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of FGF-21 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area Angiogenesis; cell growth;
Fibroblast growth factor 21(FGF-21) Background: FGF-21 (Fibroblast Growth Factor 21) is a member of an FGF subfamily that also includes FGF-19 and FGF-23. FGF21 stimulates glucose uptake in adipocytes but not in other cell types. This effect is additive to the activity of insulin. FGF21 treatment of adipocytes is associated with phosphorylation of FRS2, a protein linking FGF receptors to the Ras/MAP kinase pathway. FGF21 injection in ob/ob mice results in an increase in Glut1 in adipose tissue. FGF21 also protects animals from diet-induced obesity when overexpressed in transgenic mice and lowers blood glucose and triglyceride levels when administered to diabetic rodents. Treatment of animals with FGF21 results in increased energy expenditure, fat utilization and lipid excretion.
Serum, plasma, Cell culture supernatant
|Antigen Name||Fibroblast growth factor 21|
|Detection Range||8.2-2000 pg/ml|