Human Fetuin A ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human Fetuin ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Fetuin in human serum and plasma. An antibody specific for human Fetuin was coated on a 96-well plate. Standards and samples are added to the wells and any Fetuin present binds to the immobilized antibody. The wells are washed and biotinylated anti-human Fetuin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Fetuin present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Cancer Biomarker;

specific against

Human

detection target

alpha 2-HS glycoprotein( Fetuin A )
Background: Fetuins are members of the cystatin family of proteases. Secreted by the liver, fetuins can be found at high levels in serum. Fetuin A promotes phagocytosis of apoptotic cells and plays a role in bone development, calcium homeostasis and insulin sensitivity. Fetuin-A has been demonstrated to play an important role in free fatty acid induced insulin resistance in the liver. Increased fetuin-A in patients with pre-diabetes is associated with increased progression to diabetes and decreased reversal to normoglycemia. Hence fetuin-A is a predictor of adverse glycemic outcomes in pre-diabetes.

alias

A2HS, AHS, FETUA, HSGA

sensitivity

100 pg/ml

detection range

0.205-50 ng/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P02765