Human FABP2 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human FABP2 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of FABP2 in Human serum, plasma and cell culture media. An antibody specific for Human FABP2 was coated on a 96-well plate. Standards and samples are added to the wells and any FABP2 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human FABP2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of FABP2 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Lipid metabolism;

specific against

Human

detection target

fatty acid binding protein 2(FABP2)
Background: FABP2, also known as intestinal fatty acid binding protein (I-FABP or FABPI) and gut FABP (gFABP), is a member of the cytosolic fatty acid binding protein family. FABPs bind free fatty acids, cholesterol, and retinoids, and are involved in intracellular lipid transport. Circulating FABP levels are used as indicators of tissue damage. Some FABP polymorphisms have been associated with disorders of lipid metabolism and the development of atherosclerosis. FABP2 mediates the absorption and intracellular transport of dietary long-chain fatty acids. Genetic variations of FABP2 are implicated in obesity and Type II diabetes.

alias

FABPI, I-FABP

sensitivity

12 pg/ml

detection range

0.024-100 ng/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P02693