Human ErbB2 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human ErbB2 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of ErbB2 in human serum and plasma. An antibody specific for human ErbB2 was coated on a 96-well plate. Standards and samples are added to the wells and any ErbB2 present binds to the immobilized antibody. The wells are washed and biotinylated anti-human ErbB2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of ErbB2 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis; Cancer Biomarker; Breast Cancer Stem Cell Markers;

specific against

Human

detection target

erb-b2 receptor tyrosine kinase 2(ErbB2 )
Background: ErbB2, also called Neu and Her2, is a transmembrane glycoprotein in the ErbB family of tyrosine kinase receptors for EGF superfamily growth factors. ErbB2 is widely expressed in epithelial cells and over-expressed in a large number of breast carcinomas. ErbB2 has no identified ligands but heterodimerizes with ErbB1/EGF R, ErbB3, or ErbB4 to form higher affinity signaling complexes. ErbB2 plays roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation. The ErbB2/ErbB3 heterodimer is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2. ErbB3, ErbB2 and neuregulin are all required for formation of the sympathetic nervous system.

alias

CD340, HER-2, HER-2/neu, HER2, MLN 19, NEU, NGL, TKR1

sensitivity

4 pg/ml

detection range

8.2-2000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P04626