For the quantitative determination of Human Ephrin-A1 / EFNA1 concentration in cell culture supernates.
Human liver cells (HepG2) were grown to confluency in DMEM supplemented with 10% fetal calf serum,Collected the supernate and assayed for natural levels of EFNA1.The concentration of Human EFNA1 detected in a HepG2 cell culture supernates is 115pg/mL.
This assay recognizes both recombinant and natural Human EFNA1.The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
EFNA1Antigene: 10882-H08Hcapture antibody type: mouse mAbdetection antibody type
Unopened Kit: Store at 2 - 8°C
Opened/Reconstituted Reagents: Please refer to CoA
Solid Phase Sandwich ELISA
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
|Detection Range||23.44-1500 pg/ml|
|Alias||Ephrin-A1 / EFNA1|