The human EpCAM / TROP1 ELISA Pair Set is for the quantitative determinationof human EpCAM / TROP1. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for EpCAM / TROP1 coated on a 96-well plate. Standardsand samples are added to the wells, and any EpCAM / TROP1 presentbinds to the immobilized antibody. The wells are washed and a biotinylatedrabbit anti-EpCAM monoclonal antibody is then added, producing anantibody-antigen-antibody "sandwich". To produces color in proportion to theamount of EpCAM / TROP1 presentin the sample strepavidin-HRP and TMB substrate solution are loaded. Theabsorbances of the microwell are read at 450 nm.
EpCAMStandard (antigene): Each vial contains 50 ng of recombinant EpCAM. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 3 ng/mL is recommended capture antibody type: 0.2 mg/mL of mouse anti-EpCAM monoclonal antibody. Dilute to a working concentration of 2 µg/mL in CBS before coating (Catalog: # 10694-MM07)detection antibody type: 0.5 mg/mL of rabbit anti-EpCAM monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1 µg/mL in detection antibody dilution buffer before use. (Catalog: # 10694-R028)Lable: HRP
The minimum detectable dose of human EpCAM / TROP1 was determined to be approximately 46.8 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
5 and 15 Plates
Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
|Sensitivity||The minimum detectable dose of human EpCAM / TROP1 was determined to be approximately 46.8 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.|
|Detection Range||46.8 pg/ml|
|Alias||EpCAM / TROP-1 / TACSTD1|