Human Eotaxin-2 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human Eotaxin-2 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Eotaxin-2 in Human serum, plasma and cell culture media. An antibody specific for Human Eotaxin-2 was coated on a 96-well plate. Standards and samples are added to the wells and any Eotaxin-2 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human Eotaxin-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Eotaxin-2 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Inflammation;

specific against

Human

detection target

C-C motif chemokine ligand 24 (Eotaxin-2)
Background: Eotaxin-2, also named myeloid progenitor inhibitory factor 2 (MPIF-2), is a member of the CC chemokine subfamily and is designated CCL24. Eotaxin-2 is constitutively expressed in the jejunum and spleen. It can also be induced in the lung by allergen challenge and IL-4. Mouse Eotaxin-2 precursor protein shares approximately 58% amino acid sequence identity with human Eotaxin-2. CCL24 is a small cytokine belonging to the CC chemokine family. CCL24 interacts withchemokine receptor CCR3 to induce chemotaxis in eosinophils.[3] This chemokine is also strongly chemotactic for resting T lymphocytes and slightly chemotactic for neutrophils. Elevated levels of eotaxin-2 has been seen in patients with aspirin-exacerbated respiratory disease (AERD), such as asthma. People with lower plasma levels of eotaxin-2 have not been showing tendency to develop aspirin inducible asthma.

alias

Ckb-6, MPIF-2, MPIF2, SCYA24

sensitivity

< 1 pg/ml

detection range

1.4-1000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

O00175