The human Endoglin (CD105 / ENG) ELISA Pair Set is for the quantitative determination of human Endoglin . This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Endoglin ( CD105 / ENG) coated on a 96-well plate. Standards and samples are added to thewells, and any Endoglin / CD105 present binds to the immobilized antibody. Thewells are washed and a horseradish peroxidase conjugated mouse anti-Endoglin /CD105 monoclonal antibody is then added, producing an antibody-antigen-antibody"sandwich". The wells are again washed and TMB substrate solution is loaded,which produces color in proportion to the amount of Endoglin / CD105 present inthe sample. To end the enzyme reaction, the stop solution is added andabsorbances of the microwell are read at 450 nm.
ENGStandard (antigene): Each vial contains 120 ng of recombinant CD105. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 1000 pg/mL is recommended capture antibody type: 0.5 mg/mL of mouse anti-CD105 monoclonal antibody. Dilute to a working concentration of 2.0 µg/mL in CBS before coating (Catalog: # 10149-MM05)detection antibody type: 0.5 mg/mL mouse anti-CD105 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1.0 µg/mL in detection antibody dilution buffer before use. (Catalog: # 10149-MM11)Lable: HRP
The minimum detectable dose of human Endoglin (CD105 / ENG) was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
5 and 15 Plates
Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
|Sensitivity||The minimum detectable dose of human Endoglin (CD105 / ENG) was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.|
|Detection Range||31.25 pg/ml|
|Alias||Endoglin / CD105 / ENG|