Human ENA-78 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human ENA-78 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of ENA-78 in human serum and plasma. An antibody specific for human ENA-78 was coated on a 96-well plate. Standards and samples are added to the wells and any ENA-78 present binds to the immobilized antibody. The wells are washed and biotinylated anti-human ENA-78 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of ENA-78 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Angiogenesis; inflammation;

specific against

Human

detection target

C-X-C motif chemokine ligand 5(ENA-78)
Background: CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1 or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-β. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on human chromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodelling. CXCL5 has been also described to regulate neutrophil homeostasis.

alias

ENA-78, SCYB5

sensitivity

10 pg/ml

detection range

24.7-18000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

P42830