BioAim Scientific (Kanada)
The Bioaim Human EG-VEGF ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of EG-VEGF in Human serum, plasma and cell culture media. An antibody specific for Human EG-VEGF was coated on a 96-well plate. Standards and samples are added to the wells and any EG-VEGF present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human EG-VEGF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of EG-VEGF present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area wound healing; tumor growth; vascular disease;
prokineticin 1(EG-VEGF) Background: Endocrine gland-derived vascular endothelial growth factor (EG-VEGF), also called prokineticin 1 (PK1), is a member of the prokineticin family of secreted proteins that share a common structural motif containing ten conserved cysteine residues that form five pairs of disulfide bonds. EG-VEGF is expressed in multiple tissues including the gastrointestinal tract and steroidogenic glands. EG-VEGF has been shown to potently stimulate the contraction of GI smooth muscle.
EGVEGF, PK1, PRK1
Serum, plasma, Cell culture supernatant
|Antigen Name||prokineticin 1|
|Detection Range||13.7-10000 pg/ml|
|Alias||EGVEGF, PK1, PRK1|