The human E-Selectin / CD62E ELISA Pair Set is for the quantitative determination of human E-Selectin . This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for E-Selectin / CD62E coated on a 96-well plate. Standards and samples are added to thewells, and any E-Selectin / CD62E present binds to the immobilized antibody.The wells are washed and a horseradish peroxidase conjugated rabbitanti-E-Selectin / CD62E monoclonal antibody is then added, producing anantibody-antigen-antibody "sandwich". The wells are again washed and TMBsubstrate solution is loaded, which produces color in proportion to the amountof E-Selectin / CD62E present in the sample. To end the enzyme reaction, thestop solution is added and absorbances of the microwell are read at 450 nm.
SELEStandard (antigene): Each vial contains 75 ng of recombinant E-selectin. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended. capture antibody type: 1 mg/mL of mouse anti-E-selectin monoclonal antibody. Dilute to a working concentration of 2.0 µg/mL in CBS before coating. (Catalog: # 10335-MM04)detection antibody type: 0.6 mg/mL rabbit anti-E-selectin monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1.0 µg/mL in detection antibody dilution buffer before use. (Catalog: # 10335-R040)Lable: HRP
The minimum detectable dose of human E-selectin was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard
5 and 15 Plates
Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20°C to -80°C and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
|Sensitivity||The minimum detectable dose of human E-selectin was determined to be approximately 31.25 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard|
|Detection Range||31.25 pg/ml|
|Alias||E-Selectin / CD62e / SELE|