BioAim Scientific (Kanada)
The Bioaim Human DTK ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of DTK in Human serum, plasma and cell culture media. An antibody specific for Human DTK was coated on a 96-well plate. Standards and samples are added to the wells and any DTK present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human DTK antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of DTK present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.Research Area hematopoiesis, embryonic development, tumorigenesis;
TYRO3 protein tyrosine kinase(DTK) Background: Dtk (Sky, Tyro3, Rse, Brt), Axl (Ufo, Ark), and Mer (human and mouse orthologs of chicken c-Eyk) constitute the TAM receptor tyrosine kinase subfamily. This RTK subfamily is characterized by an extracellular domain that consists of two Ig-like motifs and two fibronectin type III motifs. These receptors bind the vitamin K-dependent protein Growth Arrest Specific Gene 6 (Gas6). Receptor activation leads to cell proliferation, migration, or the prevention of apoptosis. Cellular signaling through this family of RTKs is involved in hematopoiesis, embryonic development, tumorigenesis, and spermatogenesis.
BYK, Dtk, Etk-2, RSE, Rek, Sky, Tif
Serum, plasma, Cell culture supernatant
|Antigen Name||TYRO3 protein tyrosine kinase|
|Detection Range||40.9-10000 pg/ml|
|Alias||BYK, Dtk, Etk-2, RSE, Rek, Sky, Tif|