The human Dkk3 / REIC ELISA Pair Set is for the quantitative determination of human Dkk3. This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Dkk3 /REIC coated on a 96-well plate. Standards and samples are added to the wells,and any Dkk3 / REIC present binds to the immobilized antibody. The wells arewashed and a biotinylated rabbit anti-Dkk3 polyclonal antibody is then added,producing an antibody-antigen-antibody "sandwich". To produces color inproportion to the amount of Dkk3 / REIC present in the sample strepavidin-HRPand TMB substrate solution are loaded. The absorbances of the microwell areread at 450 nm.
DKK3Standard (antigene): Each vial contains 98 ng of recombinant DKK3. Reconstitute with 1 mL detection antibody dilution buffer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 4000 pg/mL is recommended. capture antibody type: 0.5 mg/mL of mouse anti-DKK3 monoclonal antibody. Dilute to a working concentration of 2 µg/mL in CBS before coating. (Catalog: # 10203-MM05)detection antibody type: Each vial contains 120 µg biotinylated rabbit anti-DKK3 polyclonal antibody. Reconstitute with sterile 1 mL distilled water. Dilute to a working concentration of 1 µg/mL in detection antibody dilution buffer before use.Lable: Biotin
The minimum detectable dose of human Dkk3 / REIC was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
5 and 15 Plates
Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles. Streptavidin-HRP: Store at 4°C and protect it from prolonged exposure to light. DO NOT FREEZE! It is stable for up to 6 months from date of receipt.
|Sensitivity||The minimum detectable dose of human Dkk3 / REIC was determined to be approximately 62.5 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.|
|Detection Range||62.5 pg/ml|
|Alias||DKK3 / Dkk-3 / REIC|