Human DKK1 / Dkk-1 ELISA Pair Set

Brand

Sino Biological

short description

The Human DKK1 / Dkk-1 ELISA Pair Set is for the quantitative determination of Human DKK1 / Dkk-1. The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for Human DKK1 / Dkk-1 coated on a 96-well plate. Standards and samples are added to the wells, and any Human DKK1 / Dkk-1 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-Human DKK1 / Dkk-1 monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of Human DKK1 / Dkk-1 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.

spezie

Human

detection target

DKK1
Standard (antigene): Each vial contains 20 ng of recombinant Human DKK1 / Dkk-1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20°C; to -80°C; in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 2500 pg/mL is recommended.
capture antibody type: 0.5 mg/mL of mouse anti-Human DKK1 / Dkk-1 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 1 µg/mL in PBS before coating. (Catalog: # 10170-MM08)
detection antibody type: 0.2 mg/mL of mouse anti-Human DKK1 / Dkk-1 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4°C;). Dilute to working concentration of 0.1 µg/mL in detection antibody dilution buffer before use. (Catalog: # 10170-MM03)
Lable: HRP

sensitivity

The minimum detectable dose of Human DKK1 / Dkk-1 was determined to be approximately 39.06 pg/ml. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.

Detection limitation

39.06 pg/ml

certificate

ISO9001-2008

pack size

5 and 15 Plates

storage and Stability

Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Store at 4°C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.