Human Dkk-4 ELISA Kit

Brand

BioAim Scientific (Kanada)

short description

The Bioaim Human Dkk-4 ELISA kit is a solid phase sandwich ELISA (enzyme-linked immunosorbent assay) for the quantitative measurement of Dkk-4 in Human serum, plasma and cell culture media. An antibody specific for Human Dkk-4 was coated on a 96-well plate. Standards and samples are added to the wells and any Dkk-4 present binds to the immobilized antibody. The wells are washed and biotinylated anti-Human Dkk-4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is added to the wells. The wells are again washed and TMB substrate solution is added, which produces a blue color in direct proportion to the amount of Dkk-4 present in the initial sample. The Stop Solution changes the color from blue to yellow, and the microwell absorbances are read at 450 nm.
Research Area Embryogenesis and morphogenesis;

specific against

Human

detection target

dickkopf WNT signaling pathway inhibitor 4(Dkk-4)
Background: Members of the dickkopf-related protein family (Dkk-1, -2, -3, and -4) are secreted proteins with two cysteine-rich domains separated by a linker region. Dkk-3 and -4 also have one prokineticin domain. Dkk-1, -2, and -4 function as antagonists of canonical Wnt signaling by binding to LRP5/6, preventing interaction of LRP5/6 with Wnt-Frizzled complexes. Dkk-1, -2, and -4 also bind cell surface Kremen-1 or -2 and promote the internalization of LRP5/6. Antagonistic activity of Dkk-3 has not been demonstrated. Dkk proteins have distinct patterns of expression in adult and embryonic tissues and have a wide range of effects on tissue development and morphogenesis. The Dkk family also includes Soggy, which is homologous to Dkk-3 but not to the other family members.

alias

DKK-4

sensitivity

10 pg/ml

detection range

13.7-10000 pg/ml

sample type

Serum, plasma, Cell culture supernatant

pack size

1x96 Wells

Assay time

4.5 h

Principle of the Elisa

Sandwich ELISA

SwissProtID

Q9UBT3